Vet World Vol.17 July-2024 Article - 21
Research Article
Veterinary World, 17(7): 1596-1602
https://doi.org/10.14202/vetworld.2024.1596-1602
Distribution of extended-spectrum β-lactamase producing Escherichia coli genes in an integrated poultry-fish farming system in Bogor, Indonesia
2. Bogor Agricultural Development Polytechnic, Bogor, Indonesia.
3. Division of Pathology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia.
4. Division of Veterinary Public Health and Epidemiology, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia.
5. Animal Products Quality Testing and Certification Center, Directorate General of Livestock and Animal Health, Ministry of Agriculture, Bogor, Indonesia.
Background and Aim: The excessive use of antimicrobials in livestock farming leads to the emergence and dissemination of antimicrobial-resistant organisms. This study aimed to detect extended-spectrum β-lactamase (ESBL)-producing Escherichia coli genes in integrated poultry-fish farms in Bogor, Indonesia.
Materials and Methods: A total of 256 samples were collected from six poultry-fish farms. One hundred and seventy-five chicken cloaca swabs, 60 fish skin swabs, six pond water samples, and 15 farmer’s hand swabs. ESBL-producing E. coli was confirmed through double-disk diffusion. The specific primers and probe genes for quantitative polymerase chain reaction detection of ESBL-producing E. coli targeted blaTEM, blaCTX-M, blaSHV, and blaOXA-48 genes.
Results: Among the 256 samples tested, 145 (56.6%) were positive for E. coli, and 67.6% (98/145) were identified as ESBL-producing E. coli. The most ESBL-producing E. coli isolates were obtained from chicken cloaca (78.3%, 72/92), followed by pond water (66.7%, 4/6), fish skin (47.6%, 20/42), and farmer’s hand swabs (40%, 2/5). About 100% of the isolates carried the genes blaTEM and blaCTX-M, whereas 17.3% and 24.5% carried blaSHV and blaOXA-48, respectively.
Conclusion: ESBL-producing E. coli genes were investigated in chicken cloaca, fish, pond water, and farmers’ hands within an interconnected poultry-fish farming operation. The ESBL-producing E. coli in chickens can transfer resistant genes to aquatic environments. The transfer could harm other aquatic species and food chains, potentially threatening human health.
Keywords: blaCTX-M, blaOXA-48, blaSHV, blaTEM, integrated poultry-fish farming system.
How to cite this article: Handayani KS, Setiyono A, Lukman DW, Pisestyani H, and Rahayu P (2024) Distribution of extended-spectrum β-lactamase producing Escherichia coli genes in an integrated poultry-fish farming system in Bogor, Indonesia, Veterinary World, 17(7): 1596-1602.
Received: 2024-03-24 Accepted: 2024-06-25 Published online: 2024-07-26
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.1596-1602
Copyright: Handayani, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
