Vet World Vol.17 September-2024 Article - 18
Research Article
Veterinary World, 17(9): 2104-2114
https://doi.org/10.14202/vetworld.2024.2104-2114
House cricket protein hydrolysates alleviate hypertension, vascular dysfunction, and oxidative stress in nitric oxide-deficient hypertensive rats
2. Division of Physiology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
3. Department of Food Technology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand.
4. Chulabhorn International College of Medicine, Thammasat University, Pathumthani, 12120, Thailand.
5. Thammasat University Research Unit in Physiology and Integrated Medicine, Thammasat University, Pathumthani, 12120, Thailand.
6. Department of Physical Therapy, Faculty of Allied Health Sciences, Burapha University, Chonburi, 20131, Thailand.
Background and Aim: Edible insects with high protein content and bioactive peptides with health promotion against chronic disease. Deficiency of nitric oxide (NO) contributes to hypertension, a leading cause of cardiovascular diseases and death worldwide. This study assessed the antihypertensive effects of house cricket protein hydrolysates (HCPH) in NO-deficient hypertensive rats.
Materials and Methods: Male Sprague-Dawley rats (n = 12/group) were hypertensive after the administration of Nω-nitro-L-arginine methyl ester (L-NAME) at a dose of 50 mg/kg body weight (BW)/day in drinking water for 7 weeks. The animals were then treated with HCPH (250 or 500 mg/kg BW/day) or lisinopril (Lis) (1 mg/kg BW/day) for the last 4 weeks of L-NAME administration. Blood pressure (BP), vascular function, and structural changes, endothelial NO synthase (eNOS), and p47phox nicotinamide adenine dinucleotide phosphate (NADPH) oxidase protein expression in aortic tissues, plasma nitrate/nitrite, plasma angiotensin-converting enzyme (ACE) activity, and oxidative stress markers in blood and tissues were evaluated.
Results: Induction of hypertension resulted in significantly elevated BP, decreased plasma nitrate/nitrite concentration, abolished vascular function, and increased vascular wall thickness. Overproduction of carotid and mesenteric superoxide, increased plasma, heart, and kidney malondialdehyde, and protein carbonyl levels, and increased plasma ACE activity were observed. Down-expression of eNOS with overexpression of p47phox NADPH oxidase subunit was also found in L-NAME hypertensive rats. Oral treatment with HCPH, particularly at a dose of 500 mg/kg BW/day, significantly alleviated these alterations in a manner comparable to that of Lis.
Conclusion: HCPH improved vascular function and exerted antihypertensive effects, mainly due to the improvement of NO bioavailability, reduction of oxidative stress, and inhibition of ACE.
Keywords: angiotensin-converting enzyme activity, house cricket protein hydrolysates, hypertension, nitric oxide deficiency, oxidative stress, vascular dysfunction.
How to cite this article: Sangartit W, Suwannachot P, Thawornchinsombut S, Jan-On G, Boonla O, and Senaphan K (2024) House cricket protein hydrolysates alleviate hypertension, vascular dysfunction, and oxidative stress in nitric oxide-deficient hypertensive rats, Veterinary World, 17(9): 2104-2114.
Received: 2024-03-16 Accepted: 2024-08-08 Published online: 2024-09-20
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2024.2104-2114
Copyright: Sangartit, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
