Vet World Vol.18 March-2025 Article - 19
Research Article
Veterinary World, 18(3): 731-745
https://doi.org/10.14202/vetworld.2025.731-745
β-Aminopropioamidoximes derivatives as potential antitubercular agents against anthropozoonotic infections caused by Mycobacterium tuberculosis and Mycobacterium bovis
2. National Scientific Center of Phthisiopulmonology, Ministry of Health of the Republic of Kazakhstan, National Reference Bacteriological Laboratory, Almaty, Kazakhstan.
3. LLP Scientific and Production Center BioVet, Almaty, Kazakhstan.
Background and Aim: Tuberculosis (TB) remains a significant global health challenge, with increasing incidences of drug-sensitive (DS) and multidrug-resistant (MDR) TB. In addition, Mycobacterium bovis-induced zoonotic TB (zTB) presents treatment difficulties due to its resistance to pyrazinamide and the prolonged treatment duration required. This study aims to evaluate the antitubercular potential of β-aminopropioamidoxime derivatives against DS and MDR Mycobacterium tuberculosis and M. bovis strains, and utilizing the SwissADME prognostic tool to predict the drug- and lead-likeness of the described compounds.
Materials and Methods: Six β-aminopropioamidoxime derivatives were synthesized through O-aroylation of amidoxime followed by dehydration to form 1,2,4-oxadiazoles. The compounds were tested in vitro against DS, MDR M. tuberculosis, and M. bovis using Sotton’s liquid medium and subcultured on dense Lowenstein-Jensen medium. SwissADME was used to predict drug-likeness and pharmacokinetic properties.
Results: The derivatives exhibited significant antitubercular activity, with in vitro efficacy 5–100 times greater than rifampicin. 1,2,4-oxadiazoles with para-bromo and meta-chloro substituents demonstrated the highest activity against DS and MDR M. tuberculosis, while O-para-toluoyl-β-(morpholin-1-yl)propioamidoxime salts (hydrochloride, oxalate and citrate) were 10 times more active against M. bovis. SwissADME analysis confirmed favorable pharmacokinetic properties, including high gastrointestinal absorption and drug-likeness, with lead-likeness identified in four compounds.
Conclusion: The study presents β-aminopropioamidoxime derivatives as promising candidates for antitubercular therapy against both human and zTB. Their enhanced activity, oral bioavailability, and potential integration into new treatment regimens underscore their therapeutic relevance. Further in vivo studies are recommended to validate their efficacy and safety for clinical applications.
Keywords: antitubercular agents, Mycobacterium bovis, Mycobacterium tuberculosis, SwissADME, tuberculosis, β-aminopropioamidoximes.
How to cite this article: Kayukova L, Bismilda V, Turgenbayev K, Uzakova A, Baitursynova G, Jussipbekov U, Mukanova M, Chingissova L, Dyussembayeva G, Borsynbayeva A, Yerlanuly A, and Auyezov A (2025) β-Aminopropioamidoximes derivatives as potential antitubercular agents against anthropozoonotic infections caused by Mycobacterium tuberculosis and Mycobacterium bovis, Veterinary World, 18(3): 731-745.
Received: 2024-10-11 Accepted: 2025-02-20 Published online: 2025-03-31
Corresponding author: E-mail:
DOI: 10.14202/vetworld.2025.731-745
Copyright: Kayukova, et al. This article is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/ by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/ publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
